Continue with translation. (Eucaryotes)

Continue with translation. (Eucaryotes) Online Free life science study material. Hi

Translation involves three organelles viz. Ribosome, mRNA and tRNA. Initiation, elongation and termination are three phases of translation. 1. Initiation: First step is tRNA charging. This takes place in cytosol. Similar step as in procaryotes. The difference lies in the initiation factors called eIFs. • The ribosome breaks before initiation. Smaller subunit 40S binds with eIF3 to form 40SN. eIF2 brings charged tRNA and binds to P site of ribosome and the complex becomes 43SN.In this step GDP is exchanged with GTP ,eIF2 is called GEF(guanine nucleotide exchange factor). • eI4F also called eIF4 helps in mRNA binding and forms 48SN complex. • 48SN bind with 60S sbunit to make 80S ribosome. • eIF1 causes stimulation of translation without eIF1 the 40S particle scan only few nucleotides. • eIF4A has helicase activity and unwinds any secondary structure of mRNA. • eIF4 is complex of eIF4A, eIF4E, eIF4G. adsbygoogle = window.adsbygoogle || []).push({}); • eIF4E has 5' Cap binding activity as ribosome has cap dependent entry and scans for AUG (start codon), eIF4G is adapter protein, capable of binding to eIF4E and 3'Poly A binding protein (PAB). In the next blog I will cover elongation.