Inhibitors

Free online life science study material. Hi Some inhibitors with their role. Azide - it inhibits electron transport chain in mitochondria. Atractyloside - It inhibits ATP ADP translocation. Rotenone - It inhibits electron transport chain in mitochondria. Antimycin A - inhibits electron transport chain in mitochondria. Dinitrophenol - uncoupling of electron transport and oxidative phosphorylation. Carbon Monoxide- Inhibits electrons transport chain in mitochondria. Oligomycin - It inhibits ATP Synthesis. FCCP - makes membrane permeable to proton. Malonate - Prevents oxidation of succinate. Cyanide- inhibit cytochrome oxidase.

Inhibitors of transcription: Alpha amanitin -inhibition of RNA plymerase II of eukaryotes.(Polymerase III require high concentration for inhibition and Polymerase I is not affected) Acridine and actinomycin D -intercalate between successive Guanine and Cytosine in RNA as well as DNA. Rifampicin - bind to beta subunit of bacterial RNA polymerase prevent promoter clearance step.

Inhibitors of translation: Ricin - Inactivates 60S subunit of eucaryotic ribosome. Streptomycin- Binds with 30S subunit of ribosome of bacteria and cause misreading of mRNA. Puromycin- Binds to A site of Ribosome. Erythromycin- binds to 50S of ribosome to inhibit peptide chain elongation.

Inhibitor of replication Cyclophosphamide is a cyclic phosphamide with a bis-(2-chloroethyl) group that can be metabolized by cells into phosphoramide mustard which alkylates macromolecules, including DNA,and cross-links the two DNA strands. Cisplatin (CDDP)is an inorganic complex of platinum that cross-links DNA.